Biosynthesis of phytosterols using stable isotopes (13C, 2H).
نویسندگان
چکیده
منابع مشابه
Recent highlights in biosynthesis research using stable isotopes
The long and successful history of isotopic labeling experiments within natural products research has both changed and deepened our understanding of biosynthesis. As demonstrated in this article, the usage of isotopes is not at all old-fashioned, but continues to give important insights into biosynthetic pathways of secondary metabolites. This review with 85 cited references is structured by se...
متن کاملUsing Stable Isotopes
Introduction Direct measurement of de novo lipogenesis has not previously been possible in humans. We measured de novo hepatic lipogenesis in normal men by means of stable isotopes and by combining the acetylated-xenobiotic probe technique with mass isotopomer analysis of secreted very low density lipoproteinfatty acids (VLDL-FA). Sulfamethoxazole (SMX) was administered with ['3Clacetate during...
متن کامل2H-DNP-enhanced 2H-13C solid-state NMR correlation spectroscopy.
Perdeuteration of biological macromolecules for magic angle spinning solid-state NMR spectroscopy can yield high-resolution (2)H-(13)C correlation spectra and the method is therefore of great interest for the structural biology community. Here we demonstrate that the combination of sample deuteration and dynamic nuclear polarization yields resolved (2)H-(13)C correlation spectra with a signal e...
متن کاملThe biosynthesis of picromycin using 13C enriched precursors.
Sir: Using 13C enriched precursors it has been elucidated recently1) that the aglycone of the leucomycins was derived from five acetates, one propionate, one butyrate and an unknown C2-unit corresponding to carbons-3 and -4. In the case of magnamycin which is C-9 oxidation product of leucomycin, previous studies using 14C labeled precursors2) suggested an acetate origin for carbons-3 and -4. An...
متن کاملElucidation of intrinsic biosynthesis yields using 13C-based metabolism analysis
This paper discusses the use of 13C-based metabolism analysis for the assessment of intrinsic product yields - the actual carbon contribution from a single carbon substrate to the final product via a specific biosynthesis route - in the following four cases. First, undefined nutrients (such as yeast extract) in fermentation may contribute significantly to product synthesis, which can be quantif...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Plant tissue culture letters
سال: 1990
ISSN: 1884-7706,0289-5773
DOI: 10.5511/plantbiotechnology1984.7.1